The original silver staining method is cumbersome and takes a long-time to complete. 2D-Silver Stain Reagent II was developed to provide simple, fast and highly sensitive silver staining. It is applicable to the detection of proteins and nucleic acids subjected to polyacrylamide and SDS-polyacrylamide slab gel electrophoresis.
Detection of proteins and nucleic acids migration by polyacrylamide gel electrophoresis and silver staining are now being highlighted as one of the most sensitive staining methods. However, the original silver staining method is not necessarily easy to work and takes a long-time to complete. 2D-SILVER STAIN-II is an improved reagent kit developed to provide simple and fast staining.
Features
•Rapid staining results after electrophoresis
•High sensitivity
◦For proteins: 50 to 100 times more sensitivity than CBB staining
◦For nucleic acids: 50 to 100 times more sensitivity than EB staining
•Simple preparation and simple operation
•No use of heavy metals which need to be controlled by regulations
•Staining process can be stopped any time during development for desired results
Package size and Storage
•Sufficient to stain 10 slabs gels (140 mm x 140 mm x 1.0 mm)
•Store in a dark place at 2-10°C
•Shelf life is printed on the package
| Documents & Links for 2D-Silver Stain Reagent II (for 10 gels) | |
| Datasheet | 2D-Silver Stain Reagent II Datasheet |
| Vendor Page | 2D-Silver Stain Reagent II (for 10 gels) at Cosmo Bio LTD |
| Documents & Links for 2D-Silver Stain Reagent II (for 10 gels) | |
| Datasheet | 2D-Silver Stain Reagent II Datasheet |
| Vendor Page | 2D-Silver Stain Reagent II (for 10 gels) |
| Citations for 2D-Silver Stain Reagent II (for 10 gels) – 13 Found |
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| Comparison of biological activities of human antithrombins with high-mannose or complex-type nonfucosylated N-linked oligosaccharides. Glycobiology. 2016 May;26(5):482-92. doi: 10.1093/glycob/cww001. Epub 2016 Jan 7. PubMed |
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| Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell. 2014 Dec 1;25(24):3851-60. doi: 10.1091/mbc.E14-07-1245. Epub 2014 Sep 24. PubMed |
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| Peptide-lipid huge toroidal pore, a new antimicrobial mechanism mediated by a lactococcal bacteriocin, lacticin Q. Antimicrob Agents Chemother. 2009 Aug;53(8):3211-7. doi: 10.1128/AAC.00209-09. Epub 2009 May 26. PubMed |
| Alpha2,6-sialic acid on platelet endothelial cell adhesion molecule (PECAM) regulates its homophilic interactions and downstream antiapoptotic signaling. J Biol Chem. 2010 Feb 26;285(9):6515-21. doi: 10.1074/jbc.M109.073106. Epub 2010 Jan 4. PubMed |
| Proteomic study of sera from patients with bladder cancer: usefulness of S100A8 and S100A9 proteins. Cancer Genomics Proteomics. 2010 Jul-Aug;7(4):181-9. PubMed |
| The Arabidopsis FLAKY POLLEN1 gene encodes a 3-hydroxy-3-methylglutaryl-coenzyme A synthase required for development of tapetum-specific organelles and fertility of pollen grains. Plant Cell Physiol. 2010 Jun;51(6):896-911. doi: 10.1093/pcp/pcq068. Epub 2010 May 3. PubMed |
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| Vitelline membrane proteins promote left-sided nodal expression after neurula rotation in the ascidian, Halocynthia roretzi. Dev Biol. 2019 May 1;449(1):52-61. doi: 10.1016/j.ydbio.2019.01.016. Epub 2019 Jan 30. PubMed |
| Native SAG1 in Toxoplasma gondii lysates is superior to recombinant SAG1 for serodiagnosis of T. gondii infections in chickens. Parasitol Int. 2019 Apr;69:114-120. doi: 10.1016/j.parint.2019.01.001. Epub 2019 Jan 7. PubMed |